State Road 334 in the U.S. state of Indiana was a six-mile (10 km) route in southeastern Boone County.

State Road 334 connected Interstate 65 (at exit 130) with U.S. Route 421.

Its western terminus was at Indianapolis Road just west of Interstate 65.

State Road 334 was decommissioned by the Indiana Department of Transportation in the summer of 2011 and turned over to local units of government.

The bridges along the route were turned over to Boone County.