Indicator organisms are used as a proxy to monitor conditions in a particular environment, ecosystem, area, habitat, or consumer product.
In the Great Lakes, a study was conducted testing for both fecal indicator bacteria (FIB) concentrations and pathogen gene markers.
[7] FIB were collected via membrane filtration and serial dilution methods, producing samples which could be cultured and used to run PCR and amplify the pathogenic genes in question.
[8] Common FIB were measured including enterococci which presented itself in levels as high as 242,000 MPN/100mL within onsite wastewater treatment systems.
In Texas, the occurrence and distribution of FIB, in particular fecal coliforms and E. coli, were measured in streams that receive discharge from the Dallas Fort Worth International Airport and the surrounding area.
[9] These streams receiving the waste are home to aquatic life, used for recreational purposes, and as fishing sites.
Composition of fungal communities has been found to be a good indicator of environmental properties like pH, altitude and water temperature.
Chauvet[16] used this approach to take ecosystem-wide measurements of these variables using a network of monitoring stations at 27 streams in Southwestern France.
They took many standard measures of water quality -- temperature, oxygen saturation, pH, and dissolved nitrogen, organic carbon and sulfur levels.
They found 38 fungal species, including 12 hyphomycetiae and 13 potential pathogens, belonging either to the dermatophytes or to relatives of C. albicans.
Cudowski et al. found that they could determine whether a sample of water had been taken from the natural (lake-like) or artificial part of the canal.
[22] Ascaris and whipworm that are identified in reusable wastewater systems can cause certain diseases and complications if ingested by humans and pigs.
The presence or absence of viable helminth eggs ("viable" meaning that a larva would be able to hatch from the egg) in a sample of dried fecal matter, compost or fecal sludge is often used to assess the efficiency of diverse wastewater and sludge treatment processes in terms of pathogen removal.
This type of inactivation occurs when feces is stored between 1-2 years, a high total solids content (>50-60%) is present, items such as leaves, lime, earth, etc.