I-269 currently connects to its parent route, I-69, at an interchange in Hernando, Mississippi, and will do so again in Millington, Tennessee, in the future.

The highway travels eastward across rural areas to the town of Byhalia, Mississippi, where it has an interchange with I-22/U.S.

Here, the highway continues northeastward, slowly veering north towards the Tennessee state line.

The Tennessee Department of Transportation's (TDOT) plans called for the two sections to be connected in phases.

[7] On October 18, 2007, the Mississippi Department of Transportation (MDOT) announced that a bond was successfully issued through the state's Highway Enhancements Through Local Partnerships (HELP) Program for $83 million (equivalent to $118 million in 2023[8]) to cover planning and right-of-way acquisition costs for Mississippi's portion of the route.