Isaac Makwala (born 24 September 1985) is a Botswana sprinter who specializes in the 400 metres.

His personal best time of 43.72, set 5 July 2015 in La Chaux-de-Fonds was an African continental record and ranks him 13th on the all-time list.

On July 14, 2017, Isaac Makwala became the first man in history to run a 200 m within 20 seconds and a 400 m within 44 seconds on the same day by running 43.92 in the 400, then 19.77 (no wind) in the 200, 2 hours and 20 minutes later at the Meeting de Atletismo Madrid.

[1][2] He qualified for 400m final at the 2017 World Championships, before being withdrawn from the race, due to illness.

He competed in the 400m at the 2020 Summer Olympics, finishing 7th in the final.