Her parents were Baron Domiziano de Rosis and Baroness Gabriella Francesca Berlingieri, nobles of Rossano, Calabria, Italy.
At the age of 15 Isabella consecrated herself to the Most Sacred Heart of Jesus with the Formula of Saint Margaret Mary Alacoque, promising love and reparation.
Finally, after receiving spiritual direction from her confessor, she founded the Congregation of the Reparatrix Sisters of the Sacred Heart on 24 October 1875.
She lived not for herself but for the Lord in a continuous attempt to forget herself in order to please God, always keeping her heart free from the world’s many attractions and enticements.
She occupied herself solely with the thought of serving God and the complete fulfillment of His will, in order that Jesus must reign as absolute sovereign in her heart.