Isabella[1] of Burgundy (1270 – August 1323), Lady of Vieux-Château, was the second and last Queen consort of Rudolf I of Germany.

[2] She was the second daughter of Hugh IV, Duke of Burgundy[2] and his second wife Beatrice of Navarre.

He was succeeded as Duke of Austria by his co-ruling sons Albert I and Rudolph II.

She returned to the Court of Burgundy and was granted the title of Lady of Vieux-Château on 20 November 1294.

She is not to be confused with another Isabella of Burgundy who married with Pierre IX de Chambly, Lord of Neaufles.