Isetnofret (or Isis-nofret or Isitnofret) (Ancient Egyptian: "the beautiful Isis") was one of the Great Royal Wives of Pharaoh Ramesses II and was the mother of his successor, Merneptah.

She must have married Ramesses II even before he came to the throne as her eldest children already appear in scenes from the time of Seti I.

[4] Queen Isetnofret's titles include: Hereditary Princess (iryt-p`t), Great of Praises (wrt-hzwt), King’s Mother (mwt-niswt), Mistress of the entire Two Lands (hnwt-t3wy-tm), King’s Wife (hmt-nisw), Great King’s Wife (hmt-niswt-wrt) Isetnofret is known from several inscriptions and small statues.

Most of the items and scenes mentioning Queen Isetnofret seem to be associated with her sons Ramesses, Khaemwaset and Merenptah.

[5] A daughter of her son Khaemwaset (sometimes called Isetnofret III) was named after her.