It is in the borough of Mitte and runs between Rathausstraße and Stralauer Straße, next to the Rotes Rathaus, Berlin's town hall.

As the name of the street suggests, it once ran through the Jewish district of medieval Berlin, which was no ghetto-like obligatory residential area for Jews.

An alley of Jüdenstraße named Großer Jüdenhof was the site of the synagogue, school and miqveh.

They were founded on an estate of the Margrave of Brandenburg within his immunity district (landesherrliche Freiheit) not under Berlin's city council jurisdiction.

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