Jacobs Creek is a tributary of the Delaware River in Mercer County in the U.S. state of New Jersey.

After receiving Woolsey Brook from the left, Jacobs Creek flows roughly parallel to Jacobs Creek Road (CR 637), which is on the left bank, and passes under Bear Farm Road (CR 579).

The creek turns southwest as it reaches the boundary between Hopewell Township and Ewing Township and follows this boundary, crossing it several times before passing under River Road (New Jersey Route 29), the Delaware and Raritan Canal and its towpath and entering the Delaware River.

Birds near the trail and creek may include Carolina wrens, woodpeckers, red-eyed vireos and Baltimore orioles.

Evergreens, eastern redcedars, maples, hickories, ashes, and oaks grow along the main trail.