Jacqueline was the daughter of Charles de Rohan and Jeanne de Saint-Severin, and regent of the Neufchâtel and of Valangin during the minority of her son Leonor, Duke de Longueville, Duke d' Estouteville.
Her husband, Francois of Orleans-Longueville, Marquis de Rothelin, died on 25 October 1548, and in watching her son Leonor's interests in Neuchâtel she was brought into contact with the reformers in Switzerland.
She then embraced Protestantism and turned her château at Blandy, in Brie, into a refuge for Huguenots.
[1] In 1567 she underwent a term of imprisonment at the Louvre for harbouring Protestants.
[1] On 19 June 1536, at Lyon, Jacqueline married François of Orléans-Longueville,[2] Marquis de Rothelin, Prince of Chalet-Aillon, Viscount of Melun (2 March 1513 – 25 October 1548), son of Louis I d'Orléans, duc de Longueville, Duke of Neufchatel, Prince of Chatel-Aillon and Johanna of Baden-Hochberg, Countess of Neufchatel and Margravine of Rothelin, with whom she had: