National Route 390 (国道390号, Kokudō Sanbyaku kyu-jū-gō) is both the southernmost and westernmost of the national highways of Japan.

It connects Ishigaki, Okinawa on Ishigaki Island, to Miyako-jima, and Naha, Okinawa on Okinawa Island in Japan.

Spanning the three islands by ferry, the highway has a total length of 58.2 kilometers (36.2 mi) on land and 552.2 kilometers (343.1 mi) when maritime distance is added.

National Route 390 features the 730 Intersection in Ishigaki, a monument to the day when traffic in Okinawa Prefecture was reverted from driving on the right-hand side of the road to the left after the United States relinquished control over the prefecture to Japan following the 1971 Okinawa Reversion Agreement.

This article relating to the roads and highways in Japan is a stub.