Son of a builder, Huyot attended the École nationale supérieure des Beaux-Arts in Paris, and won the Prix de Rome in 1807.
He was part of the Cléopâtra expedition under Louis Nicolas Philippe Auguste de Forbin in 1817, and Huyot sent to Jean-François Champollion a collection of inscriptions he found in the temple of Ramses II at Abu Simbel; this contribution was helpful in Champollion's work in deciphering hieroglyphics.
Huyot also provided technical archeological assistance to the Fine Arts section of the French scientific Morea expedition into Greece in 1823.
Among his architectural students were Swiss architect Melchior Berri,[1] Hector Lefuel, Alexis Paccard, Jean-Louis Victor Grisart, and Jean-Charles Danjoy.
Beginning in 1823 Huyot began presenting lectures in classical architectural history at the École des Beaux-Arts, lectures that influenced a group of self-stylized "romantic" architectural students including Félix Duban, Henri Labrouste, Léon Vaudoyer and Louis Duc.