Jefferson Prairie Settlement was a pioneer colony of Norwegian-Americans located in the Town of Clinton, in Rock County, Wisconsin, United States.

This site and the nearby Rock Prairie settlement outside Orfordville served as centers for both Norwegian immigration and developments within the Evangelical Lutheran Church in America.

Rynning's work was published as True Account of America (Norwegian: Sandfærdig Beretning om Amerika).

Pioneer Lutheran Minister, Claus Lauritz Clausen, accepted a call during 1846 from Norwegian-settlers at Jefferson Prairie.

[5] Today a historic marker near Wisconsin Highway 140, four miles south of Clinton, marks the former location of the Jefferson Prairie Settlement and highlights the role of the Nattestads in its development.