She is a member of the Latvia Fed Cup team.Ostapenko was born in Riga to former Ukrainian footballer Jevgēnijs Ostapenko (d. 2020) and Latvian-Russian tennis coach and former player Jeļena Jakovļeva.

[citation needed] At Wimbledon, Ostapenko defeated the ninth-seeded Carla Suárez Navarro in straight sets (dropping only two games in the match and grabbing her first win over a top-ten player) in the first round,[17][18] before losing to Kristina Mladenovic.

At the Birmingham Classic, she beat Anastasia Pavlyuchenkova in straight sets in the first round, and two-time Wimbledon champion Petra Kvitová in the second before she was defeated by Madison Keys in the quarterfinals.

Ostapenko made the semifinals in mixed doubles at Wimbledon with Oliver Marach, before they fell to the eventual champions, Heather Watson and Henri Kontinen.

[26] At the French Open, Ostapenko, then ranked 47th in the world, defeated Louisa Chirico, Monica Puig, Lesia Tsurenko, and Samantha Stosur.

[30] At Wimbledon, Ostapenko beat Aliaksandra Sasnovich, Françoise Abanda, Camila Giorgi, and fourth-seeded Elina Svitolina en-route to her second Grand Slam quarterfinal.

[46] At the French Open, she lost to Victoria Azarenka in the first round,[47] but reached the quarterfinals of the doubles event with Lyudmyla Kichenok, falling to Elise Mertens and Aryna Sabalenka.

[49] Despite the loss, alongside Robert Lindstedt she reached the first mixed-doubles final of her career, though they lost in straight sets to Latisha Chan and Ivan Dodig.

At Jūrmala, Ostapenko was defeated in the first round by Bernarda Pera, but she reached the final of the doubles alongside Galina Voskoboeva; the pair lost to Sharon Fichman and Nina Stojanović.

However, she went on to reach the biggest women's doubles final of her career at Beijing, partnering with Dayana Yastremska; they lost to Sofia Kenin and Bethanie Mattek-Sands.

At Linz, Ostapenko defeated Tamara Korpatsch, Alizé Cornet, and Elena Rybakina en route to reach her first semifinal appearance since 2018 Wimbledon.

At Strasbourg, she reached her first quarterfinal of the year, defeating Lauren Davis and Kiki Bertens, before losing to Nao Hibino in two tiebreak sets.

She progressed past the first round of the French Open for the first time since 2017, defeating Madison Brengle and Karolína Plíšková, before falling to Paula Badosa.

In May 2021, Ostapenko reached the semifinals in doubles at the Madrid Open with Anastasia Pavlyuchenkova defeating en route the top seeded pair and world No.

[54][55] At the 2020 Summer Olympics, Ostapenko was a flagbearer for Latvia alongside basketball player Agnis Čavars[56] and entered the singles and doubles tournaments.

[59] At the Cincinnati Open, also unseeded, she defeated Tamara Zidanšek in the first round and 13th seed Jennifer Brady (by retirement) in the second before losing to Angelique Kerber in the third.

At the Dubai Championships, she defeated four Grand Slam champions — Sofia Kenin, Iga Świątek, Petra Kvitová, and Simona Halep — en route to the final.

In Madrid, she reached the semifinals in doubles alongside Lyudmyla Kichenok, losing to eventual champions Gabriela Dabrowski and Giuliana Olmos.

In doubles with Kichenok, she reached the final, defeating Australian Open finalists Haddad Maia and Anna Danilina and top seeds Kudermetova and Mertens.

At the same tournament, she reached her first Grand Slam final in doubles with her usual partner Lyudmyla Kichenok, defeating reigning US Open champions and fourth seeds Gabriela Dabrowski and Erin Routliffe, in straight sets.

[citation needed] She reached her third singles quarterfinal at Wimbledon with wins over Ajla Tomljanović,[82] qualifier Daria Snigur,[83] Bernarda Pera[84] and Yulia Putintseva[85] before losing to eventual champion Barbora Krejčíková.

[88] In doubles at the same tournament, she reached her second Slam final for the season with her partner Lyudmyla Kichenok, defeating Anna Danilina and Irina Khromacheva in the quarterfinal,[89] and then Veronika Kudermetova and Chan Hao-Ching in the semifinal also in straight sets.

[90][91][92] Ostapenko and Kichenok defeated Kristina Mladenovic and Zhang Shuai in the final in straight sets to lift their first a Grand Slam trophy together, becoming the first Latvian and Ukrainian champions in women’s doubles at the US Open.

[94] Ostapenko and Kichenok qualified for the end-of-season WTA Finals in Riyadh, Saudi Arabia, as top seeds but were eliminated in the group stages after losing all three of their matches.

[95] Partnering Hsieh Su-wei, Ostapenko reached the doubles final at the Australian Open, losing to top seeds Kateřina Siniaková and Taylor Townsend in three sets.

In a 2016 interview, Crosscourt View labeled Ostapenko's backhand her "strongest weapon"; the same year, Hartford Courant stated that she "hits a lot of forehand winners".

[106][107][108] After putting an opponent in a vulnerable position, she regularly seeks to end the point with a cross-court forehand, a down-the-line backhand, a swinging volley, or a drop shot.

[27][109] Before her participation in the 2017 Charleston Open final, an article on the event's website declared that what was "most impressive about Ostapenko is her willingness to strike big to all corners of the court, be it a cross-court laser or a bold down-the-line winner.

However, after being coached by Marion Bartoli at Linz for the first time, her serve began to show some improvement, and in the final against Julia Görges at Luxembourg, she did not double fault once.

[122] Between 2016 and 2019, Ostapenko was the only player to have won a Grand Slam title (2017 French Open), but lost in the first round the three other times she had competed at the same tournament (2016 to Naomi Osaka, 2018 to Kateryna Baindl, and 2019 to Victoria Azarenka).