[9] The series is a reimagined take on the legendary Hanna-Barbera brand, focusing on a modernized ensemble of its characters as they live, work, and play together but have to solve the problems they have.

Huckleberry Hound is the Mayor of Jellystone, Yogi Bear is a surgeon, Cindy Bear is a genius inventor, Doggie Daddy is a lighthouse keeper who is overprotective towards his daughter Augie Doggie, Jabberjaw and Loopy De Loop work in Magilla Gorilla's haberdashery called "Magilla's", Top Cat and the Hoagy's Alley cats continue with their scam activities, El Kabong doubles as a teacher and superhero, Shazzan is a ticket seller at Jellystone Theatre and does various vendor jobs, and The Banana Splits are cartoonish criminals.

In addition, Mayor Huckleberry Hound's description for Jellystone has him advising any visitors to ignore the sign that says "Do not enter by order of the government".

Season three reveals that New Bedrock is a rival town of Jellystone that is run by Mayor Dick Dastardly and Muttley and has The Really Rottens as notable citizens.

The following gags have occurred: In contrast with previous Hanna-Barbera media, some of the established male characters are now female in the series for the sake of gender balance; these include Augie Doggie, Jabberjaw, half of Top Cat's gang, Snooper, Squiddly Diddly, and Loopy De Loop.

Greenblatt was a fan of Hanna-Barbera content as a child, and gained a deep respect for the brand from there, believing that its expansive array of characters is what really made it work.

They also managed to weave in the action-based characters, such as Jonny Quest and Shazzan, whereas many of the obscure ones, ranging from the Biskitts to Yankee Doodle Pigeon, were reduced to cameos.

Greenblatt called the series a "love letter" in an interview with Greg Ehrbar on Jerry Beck's animationscoop.com but "not a nostalgia show", though he had filled the episodes with classic cameos.