The Jesup Subdivision is a railroad line owned and operated by CSX Transportation in Georgia.

[1] The Jesup Subdivision was once a major route for the Atlantic Coast Line Railroad, one of CSX's predecessors.

From Jesup, it runs southwest in a nearly straight line through Screven, Offerman, and Blackshear to Waycross.

From Jesup to Waycross, it was originally a segment of the Atlantic and Gulf Railroad and was built in 1859 just before the start of the American Civil War.

[5] The line through Waycross was the main route for trains travelling from the northeast to Florida until the construction of the Plant System's Folkston Cutoff (which is the present-day Nahunta Subdivision).

Two local passenger trains from Savannah east to Montgomery, Alabama ran the line daily between Jesup and Waycross.

In 1980, the Seaboard Coast Line's parent company merged with the Chessie System, creating the CSX Corporation.