She is a main cast member on the animated series SpongeBob SquarePants, in which she voices Karen Plankton.

[4] Since 1999, Jill Talley has played Karen Plankton on SpongeBob SquarePants.

She was promoted to a main cast member in the credits of its 2004 film adaptation.

Talley's other vocal work includes playing several characters on Camp Lazlo (Gretchen, Nina, and Ms. Mucus) and playing Sarah Dubois on the Adult Swim show The Boondocks.

She also voiced Lady Granite in the Plastic Man DC Nation Shorts and Maja the Sky Witch in the Adventure Time episode "Sky Witch”.