On a subsequent trip to Italy he met and married Mathilde Pauline Baroness des Granges (c. 1801– 9 July 1881).
The Euphrates expedition continued, but the Helfers left it at Balis in May, after sending two crates of specimens to the British Museum (lost at sea).
Parting from Chesney the Helfers traveled from Syria to the Persian Gulf to begin the six-week voyage to Calcutta.
They stopped in Baghdad and Bushehr, and then in Muscat (Oman), arriving in Calcutta on 20 August 1836 where they stayed with acquaintances of Chesney and later at the Chowringhee Boarding House.
The Helfers left Calcutta on 21 January 1837 aboard the Elizabeth and travelled down the River Hooghly and across the Bay of Bengal to Moulmein.
Sometime during 1837, the Helfers started a plantation near Myeik focusing on palm trees, coffee and nutmegs and in 1837 and 1838 he sailed the shores of India and Burma.
Helfer under the title Reliquiae Helferianae e Sectione botanica Musei Nationalis Pragae editae anno 1937.