John Aylworth (by 1516 – 28 December 1575), of Wells, Somerset, Dartington, Devon and Bangor Place, Holborn, London, was an English politician.
Aylworth married Elizabeth Ashton, and they had six sons and one daughter.
Two of their sons, Ashton and Edward, became Members of Parliament.
He was a Member (MP) of the Parliament of England for Wells in 1547, 1553, 1558, 1559, 1563, 1571, 1572 and for Penryn in October 1553.
This article about a member of the parliament of England during the second Parliament of Elizabeth I, 11 January 1563 to 2 January 1567 is a stub.