John Gainsford (died 1543) was an English landowner and courtier.
He was the son of John Gainsford (died 1491) and Anne Worsley.
Some stained glass with his heraldry survived in the hall at Crowhurst, with the feather device of the Prince of Wales.
[1] His great uncle, Nicholas Gaynesford, had been an usher to Elizabeth of York.
[3] Gainsford married six times and had over twenty children.