John Lewis Brenner (February 2, 1832 – November 1, 1906) was an American farmer, nurseryman, and businessman who served two terms as a member of the United States House of Representatives from Ohio from 1897 to 1901.

John Brenner worked on his father's farm in the summer and attended the local public schools in the winter.

John L. Brenner was elected a member of the City of Dayton board of police commissioners, serving from 1885 to 1887.

Ohio's third district was at the time evenly divided between the two parties, and Mr. Brenner's plurality at each election was barely 100 votes.

After his congressional service, John Lewis Brenner returned to Dayton and resumed his former occupation as a dealer in leaf tobacco.