[1] Coming to divide his time between London and Tunbridge Wells, Mayo resided at the latter during the summer months, where he was the leading physician.
On resigning his hospital appointments in 1817, he settled at Tunbridge Wells, and dying 29 November 1818, was buried at Speldhurst, Kent.
[1] Mayo published The Information and Complaint made to the Court at the Hospital for the Maintenance and Education of exposed and deserted Children, 1790.
[2] His eldest son Thomas published Remarks on Insanity, founded on the Practice of John Mayo, M.D., 1817.
His second wife was Frances Lavinia, daughter of William Fellowes of Ramsey Abbey, Member of Parliament for Ludlow and Andover.