John O'Donohoe (April 18, 1824 – December 7, 1902) was a Canadian politician.

Born at Tuam in County Galway, Ireland, O'Donohoe was educated at St. Jarlath's College.

O'Donohoe ran unsuccessfully for the Legislative Assembly of Ontario in the 1871 election and in an 1872 by-election.

He ran for the House of Commons of Canada in the 1872 federal election for the riding of Toronto East and was defeated.

He was appointed to the Senate on the advice of John Alexander Macdonald in May 1882 representing the senatorial division of Erie, Ontario.