John Osborne Whitehouse (July 19, 1817 – August 24, 1881) was a U.S. Representative from New York.

He received a common-school education and moved to New York City in 1835, where he worked as a clerk.

He moved to Poughkeepsie, New York, in 1860 and continued the shoe manufacturing business.

He served as chairman of the Committee on Reform in the Civil Service (Forty-fourth Congress).

This article incorporates public domain material from the Biographical Directory of the United States Congress