Stoffolano was the first to discover the eye worm parasite (Thelazia) found in dairy cattle in the United States and published the paper with the nematologist MayBelle Chitwood.

Following graduation from Oneonta College, Stoffolano taught 8th grade English and Math for one half a year at Oppenheim-Ephratah-St. Johnsville Consolidated School.

While at the University of Massachusetts, Stoffolano extended his research to understanding more about the behavior and blood feeding of the greenhead horsefly, Tabanus nigrovittatus, which is a constant nuisance along the Atlantic Coast.

His interest in fly anatomy resulted in the important discovery that the cuticular abdominal placques on certain adult Diptera were key to the evolution of specific dipterous groups.

During his career, he was responsible for research demonstrating that adult houseflies could vector-store pathogens in their diverticulated crop for diseases such as cholera, trachoma, the ORF virus, and Escherichia coli.

[12] During his 2010 sabbatical, Stoffolano and his wife Susan traveled around the world, visiting various countries and doing research in South Africa with the specialist on adipokinetic hormones in insects, Gerd Gade.