John Whitney Barlow (June 26, 1838 – February 27, 1914) was a career officer in the United States Army.

He supervised the defenses of Nashville and was brevetted as a lieutenant colonel for his gallant service there in December 1864.

In 1872, his expedition party was attacked by about 1000 warriors led by Sitting Bull, but they were defeated by the 400 cavalry troopers assigned to guard the explorers.

He was the senior American member of the international commission that re-marked the disputed boundary with Mexico in 1892–96.

[5] While he was serving as Northwest Division Engineer based in New York City, his wife died of bronchial pneumonia at their residence in The Albany, Broadway and 51st Street, Manhattan.

His body was shipped back to the United States and was interred at Arlington National Cemetery beside his first wife.