John Wemyss (c. 1579–1636), also spelled Weemes or Weemse, was a Church of Scotland minister, Hebrew scholar and exegete.
John Wemyss was born at Lathocker in eastern Fife, and educated at the University of St Andrews.
For several years Wemyss acted as a representative of Presbyterian ministers in altercations with champions of episcopacy, for example at the Falkland Conference (4 May 1609) and the Perth Assembly of 1618 which issued the Five Articles.
After appearing before the Court of High Commission in 1620 for disobeying the Articles, he apparently gave up ecclesiastical affairs and devoted himself to study and writing.
Although his attitude to the Jews included both negative and positive views, he did express a hesitancy about persecuting them; this marked the start of the move to enlightenment tolerance.