[5] The presence of Johnston's organ is a defining characteristic which separates the class Insecta from the other hexapods belonging to the group Entognatha.
[7] In the mosquito, the Johnston's organ houses ~15 000 sensory cells in males,[8] comparable to that in the human cochlea,[9] and approximately half as many in females.
[2][11] The Johnston's organ of fruit flies, chironomids or mosquitoes can be used to detect air vibrations caused by the wingbeat frequency or courtship song of a mate.
[13][14] Insects, such as fruit flies and bees, detect sounds using loosely attached hairs or antennae which vibrate with air particle movement.
Kinematic data measured from hovering moths during steady flight indicate that the antennae vibrate with a frequency matching wingbeat (27 Hz).
During complex flight, however, angular changes of the flying moth cause Coriolis forces, which are predicted to manifest as a vibration of the antenna of at about twice wingbeat frequency (~60 Hz).
[15] Dancing honeybees (Apis mellifera) describe the location of nearby food sources by emitted airborne sound signals.
When the antennae were prevented from moving at the joints containing the Johnston's organ, bees no longer responded to biologically relevant electric fields.