Justine Johnston (June 13, 1921 – January 13, 2006) was an American film, television, and musical theatre actress.

She was occasionally mistaken for Justine Johnstone, a similarly named silent film actress, who is not her mother and with whom she had no connection.

Johnston performed throughout the Mid-Pacific during World War II.

She appeared on Broadway in such musicals as the original production of the Tony-winning Follies (by Stephen Sondheim), as well as the American production of Me and My Girl, and a revival of Irene, starring Debbie Reynolds.

Johnston served on the governing body of the Actors' Equity Association for 39 years.