In molecular biology, kanamycin nucleotidyltransferase EC 2.7.7.- (KNTase) is an enzyme which is involved in conferring resistance to aminoglycoside antibiotics.

It catalyses the transfer of a nucleoside monophosphate group from a nucleotide to kanamycin.

This enzyme is dimeric with each subunit being composed of two domains.

The C-terminal domain contains five alpha helices, four of which are organised into an up-and-down alpha helical bundle.

Residues found in this domain may contribute to this enzyme's active site.