Karl Spiro (24 June 1867 – 21 March 1932) was a German biologist, and physical chemist.
He later served as an assistant to Oswald Schmiedeberg and Franz Hofmeister at the University of Strasbourg, where in 1912 he became an honorary professor.
From 1919 to 1921 he worked as a pharmacologist in the research laboratories of Sandoz AG (Basel).
In 1921 he succeeded Gustav von Bunge as professor of physical chemistry at the University of Basel, where he also served as director of the institute for physiological chemistry.
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