The sanctuary is centered on the historic Karnala Fort and lies just off the Mumbai-Pune national highway to Goa.

Regular buses at an interval of 30 minutes are available from Panvel Bus Stand from 5:00 am to 8:00 pm.

The bird sanctuary is a popular destination for bird-watchers and hikers in the Mumbai area.

[3] Following eight species endemic to Western Ghats has been found in Karnala: Grey-fronted Green-pigeon (Treron affinis), Nilgiri Woodpigeon (Columba elphinstonii), Malabar (Blue-winged) Parakeet (Psittacula columboides), Malabar Grey Hornbill (Ocyceros griseus), White-cheeked Barbet (Megalaima viridis), Malabar Lark (Galerida malabarica), Small Sunbird (Leptocoma minima) and Vigor's Sunbird (Aethopyga vigorsii).

[3] Five rare birds, the ashy minivet, three-toed kingfisher, Malabar trogon, Slaty-legged Crake (Rallina eurizonoides), and Rufous-bellied Eagle (Lophotriorchis kienerii) have been sighted here.