Kentucky Route 1927 (KY 1927) is a 16.397-mile-long (26.388 km) east–west state highway located in and around the cities of Lexington and Winchester, Kentucky.

The western terminus of the route is at Kentucky Route 4 (East New Circle Road) east of downtown Lexington.

The eastern terminus is at Kentucky Route 627 in downtown Winchester.

In rural western Clark and eastern Fayette this road is considered one of the most dangerous because of its curves and trees.

Many fatal accidents on this road has occurred more than any other major highway, road, or street in Winchester and Lexington.