It traverses portions of northern Madison, Clark, and southern Bourbon counties.

The state highway continues northward to pass near Fort Boonesborough State Park (KY 388 provides access to that destination) and enters Clark County.

The state highway goes further north to enter Bourbon County and into the city of Paris, where it terminates in downtown at a junction with a one-way couplet in US 68 Business.

[4] The routing of KY 627 from near Fort Boonesborough to Paris, along with much of KY 388 in Madison County was originally part of US 227 when it existed from 1928 until the U.S. Route's 1972 decommissioning.

The KY 627 designation was assigned by the Kentucky Department of Highways the same year as the decommissioning of US 227.