The highway connects mostly rural areas of Boyle and Lincoln counties with Parksville, Junction City, and Stanford.

It curves to the east-northeast and enters Alum Springs, where it intersects the northern terminus of KY 3365 (Hogue Hollow Road).

[1] KY 300 turns left onto Knob Lick Road and resumes its trek to the southeast and crosses over Knoblick Creek.

Then, the highway curves back to the southeast and crosses over Hanging Fork Creek.

KY 300 travels to the north-northwest and meets its eastern terminus, an intersection with US 150 (Wilderness Road).