It is known for making early capital investments in startups such as Impossible Foods, Instacart, Affirm, DoorDash, Square and OpenAI.

[2] Khosla Ventures's first two investment vehicles were funded with his own personal capital and were not open to institutional investors.

[5] As of October 2015[update], Khosla Ventures was one of the five largest and most active investors in the space sector.

Khosla Ventures stated that the funding would be used for nuclear fusion, humanoid robots and artificial intelligence to support industries such as healthcare and transportation.

[12] In October 2024, the firm raised a special purpose vehicle of $405 million to invest in OpenAI.

The firm also invested in Instacart in 2012 as part of the seed round before going public in 2020 with $11 billion in market capital.

[15][16][17] Khosla Ventures invested in Block, Inc.'s Series A funding round in 2009 before the company went public in 2015.

[26] Through a Khosla-backed SPAC, Nextdoor, trading under the ticker KIND, had its initial public offering in November 2021 with a $4.3 billion valuation.