He was CEO and CSO, ToolGen, Inc., is a professor in the Department of Chemistry of Seoul National University and director of the Center for Genome Engineering.

ToolGen is a biotech company based on CRISPR technology focusing on human therapies, and molecular breeding in plants and animals.

From 2014, he became the founding director of the Center for Genome Engineering under the Institute for Basic Science (IBS).

[6] In 2015, Kim's IBS Center, Seoul National University, and ToolGen jointly published a paper in Nature Methods outlining their technique named Digenome-seq which locates on-target and off-target sequences in CRISPR-Cas9.

[7] Dignome-seq complements other genome wide off target analysis assays such as CIRCLE-seq and guide-seq, which are performed in vitro and in cellulo, respectively.