[1] The hospital is located in the town of Kisiizi in Rukungiri District, in the Kigezi sub-region of the Western Region of Uganda.

Kisiizi Hospital is approximately 43 kilometres (27 mi) south of Rukungiri, where the district headquarters are located.

[2] This location is also approximately 42 kilometres (26 mi) north of Kabale, the largest city in the Kigezi sub-region.

[4] The hospital is a private non-profit institution with a bed capacity of 250 plus 10 cots/incubators in the Special Care Baby Unit.

[1][5] Kisiizi Hospital was founded by missionary John Sharp in 1958 and was later taken over by the North Kigezi Diocese of the Church of Uganda.