[1] It was at the university when he was introduced to Kitasato Shibasaburō, one of Robert Koch's successors, who was a world-famous Japanese scientist studying the bacteriology and immunology of deadly disease at the time.

[1] While working for the institute, Shiga became famous for the discovery of Shigella dysenteriae, the organism that causes dysentery, in 1897, during a severe epidemic in which more than 90,000 cases were reported, with a mortality rate approaching 30%.

[1] From 1929 to 1931, Shiga was the president of Keijō Imperial University in Keijo (Seoul) and was senior medical advisor to the Japanese Governor-General of Korea.

[3] After graduating from the Tokyo Imperial University school of medicine, Kiyoshi Shiga began his career as an assistant to Kitasato Shibasaburō at the Institute for the Study of Infectious Diseases.

While studying many infamous diseases at the time, Kitasato and his colleagues, including Shiga, turned their focus to discovering the microorganism that caused the dysentery outbreak in 1897.

[4] Shiga had done research on other topics aside from Shigella dysenteriae including tuberculosis, leprosy, and beriberi throughout his career and grew an interest in chemotherapy and immunology.

[6] After the Shigella dysenteriae discovery, Kiyoshi Shiga worked with Paul Ehrlich in Europe on discovering chemotherapy methods for a blood diseases called trypanosomiasis which was caused by a protozoan microorganism.

[8] Shiga and Ehrlich's research into chemotherapy dyes lead them to the discovery of trypan red, a drug that was proven to have an effect on trypanosomiasis.

[8] In 1905, after the discovery of trypan red, Shiga returned to Japan with an international reputation and continued his work at Kitasato's laboratory.