In 1856, St. Vincent's School moved to East 2nd Street and Second Avenue, a plot of land that once belonged to Washington Irving.

The Board of Regents of the University of the State of New York granted the school a charter in 1896.

Today, the annex houses the offices of the President, Development, Recruitment & Admissions and the Christian Brothers' residential community.

In 1997, La Salle received accreditation from the Middle States Association of Colleges and Schools and in 1998 celebrated its 150th anniversary.

The school's Glee Club, which was directed by Phil Carney, performed for Martin Luther King Jr. at New York City Hall in 1965.