Lake Timberline is an unincorporated community and census-designated place (CDP) in St. Francois County, Missouri, United States.

The community is built around several reservoirs built in the valleys of Bee Run and Primrose Creek, southwest-flowing tributaries of the Big River, which in turn flows northwest to the Meramec River.

It is northwest of U.S. Route 67, 6 miles (10 km) north of Bonne Terre, 19 miles (31 km) southwest of Festus, and 53 miles (85 km) southwest of St. Louis.

The community was first listed as a CDP prior to the 2020 census.

This Saint Francois County, Missouri state location article is a stub.