Lambton House is a historic former inn in Toronto, Ontario, Canada.

It is the last remaining building from the former village of Lambton Mills along the Humber River.

The building, and most of the surrounding land, was owned by William Pearce Howland, Ontario's second Lieutenant Governor and a Father of Confederation.

[1] Located on Dundas Highway, a major route in the late 1800s, the hotel was quite busy, and it became a popular picnic spot around the turn of the century.

The building is owned by the City of Toronto and operated as an Interpretive Centre for the Lower Humber River and environs by Heritage York, a local non-profit group.