Larisaikos' first official matches took place during the 1932–33 season in the Cup of the Cities under the umbrella of Thessaly Football Clubs Association.

The club was inactive from 1933 until 1938 returning to action just two years before Greece entered the WW II.

They also qualified to the Beta Ethniki in the 1959–60, which was the second tier of Greek football finishing in the penultimate position of the table against more experienced teams like Niki Volou and Fostiras.

Larisaikos finished 8th the next season in Beta Ethniki with local rivals Niki Volou winning the group.

In 1964, Larisaikos was finally relegated from Beta Ethniki and subsequently was merged, ceasing operations after 34 years.

After the 1969 re-establishment they play their home games in Viologiko Ground,[9] located in their historic district of Nea Smirni.