The Last Supper (1445–1450) is a fresco by the Italian Renaissance artist Andrea del Castagno, located in the refectory of the convent of Sant'Apollonia, now the Museo di Cenacolo di Sant'Apollonia, and accessed through a door on Via Ventisette Aprile at the corner with Santa Reparata, in Florence, region of Tuscany.

Painted with a careful attention to naturalistic detail—a sense of real space and light, seemingly tangible details of the setting, and lifelike figures—the work must have spoken forcefully of the continued significance of the Eucharistic meal in their own world.

[3] Saint John's posture of innocent slumber neatly contrasts with Judas's tense, upright pose and exaggeratedly pointed facial features.

This work, located in the refectory of a convent of cloistered nuns, may have been seen by Leonardo da Vinci before he painted his own far more emotional Last Supper.

The contemporary fresco by Castagno on the top register, not protected, shows more degradation, and depicts the Crucifixion, flanked by the Resurrection and Burial of Christ.