The Ledbury and Gloucester Railway, (also known as the Daffodil Line), was a railway line in Herefordshire and Gloucestershire, England, running between Ledbury and Gloucester.

[1] Most of the line followed the route of the southern section of the Herefordshire and Gloucestershire Canal, which was inaugurated in 1798.

After a period of financial struggle, the canal was leased to the Great Western Railway (GWR) in 1863.

[2] The line followed a south and then south-easterly route between Ledbury railway station and Gloucester Central railway station, it joined the Gloucester to Newport Line at Over Junction.

Notably, a skew bridge that carried the line over Hereford Road in Ledbury remains in use as part of the Ledbury Town Trail footpath.