In 1748 she married James Wardrop, a Scottish immigrant who made a fortune as a merchant in Upper Marlboro, Maryland.

[2] She and her husband owned and managed Darnall's Chance, a large plantation in Upper Marlboro.

[2][3] The plantation included a large brick house, outbuildings, orchards, an ornamental garden, and a farm for livestock.

[2] They enslaved thirty-two people on their plantation, including house slaves, craftsmen, and field hands.

[4] Following her husband's death in 1760, Lee married a second time to Dr. Adam Thompson, a Scottish physician who created the American Method of smallpox inoculation.