In addition to its traditional campus in Gaffney, the university provides online degrees and previously had physical locations in Charleston, Columbia, Greenville, and Florence.

Additionally, in addition to free, reasonable accommodations through the Accessibility Office, Limestone University offers a comprehensive support program, LEAP (Learning Enrichment & Achievement Program), for Day Campus students with learning and attention challenges.

Limestone offers competitive opportunities at the NCAA Division II level for men in football, soccer, basketball, baseball, wrestling, lacrosse, golf, cross country, tennis, and track and field and for women in golf, volleyball, basketball, softball, tennis, soccer, cross country, lacrosse, cheerleading, track and field, acrobatics/tumbling, and field hockey.

Clubs and organizations at the university include academics, religious, leadership, musical, theatre, and special interest affiliations.

Students also contribute to a yearbook and a literary magazine of poems, essays, short stories, and art.