Limestone maintains CC membership in two sports, specifically men's wrestling and women's acrobatics & tumbling.

Men's wrestling is one of two sports in which the SAC and CC operate as a single league, the other being women's field hockey.

Tucker beginning in 2015 became the winningest active coach in NCAA Division II women's lacrosse.

[7] Limestone's women's basketball program has made 5 consecutive appearances in the NCAA Division II National Tournament (2012-2016), reaching the elite eight in 2014, and the final four in 2015.

[8] Bobby James, previously the defensive coordinator at Wingate University was named the inaugural head coach on December 14, 2012.

James left the program in early 2016, and on May 11, 2016, Limestone College announced the hiring of former National Football League wide receiver Mike Furrey as the second head coach for the Saints.

[10] The Saints had competed in football as an independent, but during the 2015–2018 seasons arranged a scheduling agreement with the South Atlantic Conference to provide most of their contests.

In April 2017 the Limestone Saints were announced[11] as the first associate member of the South Atlantic Conference (in any sport), and began competition in the SAC beginning in fall 2017.