The following is a list of state highways in Missouri.

State highways in Missouri are the responsibility of the Missouri Department of Transportation.

This is a list of former highways as assigned in 1922.

All numbers from Route 1 to Route 72 were issued.

In 1926, the U.S. Highway System was created and many of the highways listed below became part of a new U.S. Highway; in some cases, a highway's number was changed so as not to conflict with a U.S. Highway number (or, later, an Interstate Highway number) which came through Missouri.