Little Island is an island in the Australian state of South Australia located in Spencer Gulf off the east coast of Jussieu Peninsula on Eyre Peninsula approximately 28 kilometres (17 mi) south-east of Port Lincoln.

It was named by Matthew Flinders in memory of John Little who was one of the eight crew lost from a cutter that capsized sometime after being launched from HM Sloop Investigator to search for water on 21 February 1802.

[1] Little Island was formed about 8400 years ago when sea levels rose at the start of the Holocene.

[1] The island was one of several first sighted by Europeans on Saturday, 20 February 1802, from HMS Investigator whilst under the command of Matthew Flinders entered what is now Spencer Gulf.

[7] It first obtained protected area status as a fauna conservation reserve declared under the Crown Lands Act 1929-1966 on 16 March 1967.